Reproduction Abstracts

Few studies have evaluated sperm function and intracellular calcium levels when storage of semen for a long period of time. We have assessed sperm viability, morphology, motility and intracellular calcium levels in fish semen after a prolonged storage period. Semen samples from Atlantic salmon were preserved at 4 °C for 25 days and then evaluated for viability. The motile sperm was determined using the Image J-CASA program. To measure the calcium content, the sperm were l...

Sperm cells are complicated in vitro models. Its viability is limited, and its physiology is complex. The study of their properties is of great application in the animal production industry. Therefore, it is important to have viable and functional gametes. Consequently, it has been demonstrated that the decrease of sperm cells viability is related with the increase of the reactive oxygen species (ROS) and ROS is secondary to normal metabolic processes of the cell. One...